Alex Kenyon and Liam McAlinden have been forced to isolate due to COVID-19.
The two Shrimps players missed the trip to Maldon and Tiptree in the Emirates FA Cup at the weekend after going into self-isolation.
"Unfortunately, we have lost another two players," said boss, Derek Adams.
"We're following government guidelines and EFL protocols, Harry Davis and Cole Stockton both returned to the squad early last week after their periods of isolation so to lose another two players so soon is not ideal but we are operating in difficult times."
The pair will be unavailable for the Shrimps' upcoming Sky Bet League Two fixture versus Stevenage.